Nucleic Acid Extraction Techniques
Phenol–chloroform extraction is widely used. The process consists of vigorous mixing of phenol–chloroform solution and sample followed by centrifugation [ 7 ]. Phenol does not completely inhibit RNase activity, and this characteristic enables isolation of nucleic acid by combination with chloroform and alcohol [ 12 ].
Density Gradient-Based Nucleic Acid Isolation | SpringerLink
This procedure should remove any phenol that may have remained in the DNA sample. 6. Centrifuge at 3000× g for 5 min or until the aqueous layer is clear. Transfer the aqueous layer to a fresh Falcon tube and add an equivalent quantity of isopropanol. 7. Remove the supernatant after centrifuge at 10,000× g for 5 min. 8.